Table 1. Primers used for RT-PCR amplification of the L (partial) gene and the N gene of TOSV*.
| Primer | Sequence | PCR |
|---|---|---|
| NPhlebo1+ | 5´ 2047ATGGARGGITTTGTIWSICIICC2069 3´ | L gene–1st |
| NPhlebo1– | 5´ 2600AARTTRCTIGWIGCYTTIARIGTIGC2575 3´ | L gene–1st |
| NPhlebo2+ | 5´ 2074WTICCIAAICCIYMSAARATG2094 3´ | L gene–2nd |
| NPhlebo2– | 5´ 2318TCYTCYTTRTTYTTRARRTARCC2296 3´ | L gene–2nd |
| TosS1+ | 5´ 4CAGAGATTCCCGTGTATTAAAC25 3´ | N gene–1st |
| TosS1– | 5´ 1052GAGTGCTGCCAAGTCTTATGAC1031 3´ | N gene–1st |
| TosS2+ | 5´ 4CAGAGATTCCCGTGTATTAAACAAAAGC31 3´ | N gene–2nd |
| TosS2– | 5´ 1004TAGAGAAACTGCTCTTTCCACC983 3´ | N gene–2nd |
*RT-PCR, reverse transcription–polymerase chain reaction; TOSV, Toscana virus.