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. Author manuscript; available in PMC: 2013 May 25.
Published in final edited form as: Cell. 2012 May 25;149(5):1060–1072. doi: 10.1016/j.cell.2012.03.042

Figure 1. Erastin-induced death triggers the accumulation of cytosolic ROS whose production can be inhibited by DFO.

Figure 1

(A) Visualization of HT-1080 cell viability over time +/− erastin (Era, 10 µM) and deferoxamine (DFO, 100 µM). (B,C) Cytosolic and lipid ROS production assessed over time (2,4 and 6 hrs) by flow cytometry using H2DCFDA and C11-BODIPY. (D) Mitochondrial ROS assessed in HT-1080 cells treated for 6 hrs with erastin+/−DFO, as above, or with rotenone (250 nM)+/−DFO. In (A–D) representative data from one of four experiments is shown. (E) Erastin-induced death in 143B ρ0 and ρ+ cells. (F) mtDNA-encoded transcript levels in ρ0 and ρ+ cells. Results in (E) and (F) are mean+/−SD from one of three representative experiments.