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. Author manuscript; available in PMC: 2012 Nov 30.
Published in final edited form as: Nature. 2012 May 13;485(7400):642–645. doi: 10.1038/nature11089

Figure 2.

Figure 2

PRDM9 redirects DSBs away from functional genomic elements. a. In Prdm9−/− mice DSBs are formed at functional genomic elements marked by H3K4me3 (such as promoters, red boxes). These sites are refractory to DSB formation in wild type mice, where breaks are redirected to PRDM9-dependent H3K4me3 marks (*). b. Most DSBs occur in regions around TSSs in Prdm9−/− mice but not in wild type mice. c. The centre of Prdm9−/− hotspots at TSS coincides precisely with the H3K4me3 mark on the +1 nucleosome (red circle: −1 nucleosome; white circle: nucleosome free region; green circle: +1 nucleosome). Mean H3K4me3 coverage is grey, mean forward strand ssDNA coverage is blue and reverse strand coverage is red. The intersection point of the forward and reverse strand coverage is the mean hotspot centre (Supplementary Fig. 11).