Fig. 3.

Formation and innervation of ectopic muscles in HSA-β-cat^flox(ex3)/+ mice. (A) Schematic of left hemi-diaphragm with ectopic muscles. Square frame indicates parts of diaphragms analyzed in B-D. D, dorsal; V, ventral; M, medial; L, left. (B-D) Staining of diaphragms of different genotypes at indicated ages (NF/synaptophysin, green; AChR, red). White dashed lines indicate the edge of diaphragms. Red dashed lines encircle ectopic muscles. Arrowheads indicate extensive and longer secondary branches. Arrows indicate secondary nerve branches. (E) Enlarged image of an ectopic muscle. Arrow, ectopic axons; arrowheads, AChR clusters.