Fig. 6.
Impaired synaptic transmission in HSA-β-catflox(ex3)/+ NMJs. (A) Representative superimposed mEPP sample traces. Traces were recorded from neonatal mice at 2 mM Ca2+ (1-second × 600 traces). (B) Reduced mEPP frequency in HSA-β-catflox(ex3)/+ NMJs (**P<0.01, n=6, t-test). (C) mEPP amplitude was not changed at mutant NMJs (P>0.05, n=6, t-test). (D) Representative mEPP traces in response to 0.5 M sucrose. Shown are traces 7 minutes after the addition of sucrose. Time scale: 1 second upper panel; 1 minute lower panel. (E) Quantitative analysis of hypertonic evoked mEPP frequency shown in D (P>0.05, n=5, repeat measure). (F-J) No change in synaptic proteins in HSA-β-catflox(ex3)/+ muscle lysates. Data are mean ± s.e.m. (P>0.05, n=3 each group, t-test).