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Typical growth inhibition in liquid culture of virus constructs. Cultures of S. solfataricus GΘ infected with wild-type SSV1, diamonds, SSV-ΔVP2, triangles and SSV-Δd244, squares, were diluted in YS media to equal starting OD600 nm and incubated at 75°C. At the indicated times, samples were removed and the OD600 nm was determined and the presence of virus was confirmed in each culture via halo assay. After 96 h, 1 mL of cells were diluted 1:100 in fresh YS media and returned to 75°C.
