Table 2.

Oligonucleotides used in this work.

Name	Sequence	Description
VP2 LIPCR F	5′-CAC CGC AAG TAG GCC-3′	Flanks VP2 gene for deletion
VP2 LIPCR R	5′-CAC CCA CTT CAT ATC ACT CC-3′	Flanks VP2 gene for deletion
d244 LIPCR F	5′-ATC CAT TTA CCA TAA TCC ACC-3′	Flanks ORF d244 for deletion
d244 LIPCR R	5′-GGA AAA TGA TAT TCA ACT CAG AGG-3′	Flanks ORF d244 for deletion
b129 LIPCR F	5′-AGT TAG GCT CTT TTT AAA GTC TAC C-3′	Flanks ORF b129 for deletion
b129 LIPCR R	5′-TGA CTC CGT CAT CCT CTA AC-3′	Flanks ORF b129 for deletion
VP2 Check F	5′-ATT CAG ATT CTG WAT WCA GAA C-3′	Amplifies VP2 gene and flanking sequences
VP2 Check R	5′-TCS CCT AAC GCA CTC ATC-3′	Amplifies VP2 gene and flanking sequences
d244 Check F	5′-GGA ACT CCT CTC ATT AAC C-3′	Amplifies ORF d244 and flanking sequences
d244 Check R	5′-GAT CAT CAA CGA GTA TAT TGA CC-3′	Amplifies ORF d244 and flanking sequences
b129 Check F	5′-ATG AAG GCT GAG GAA ACA ATC GTG-3′	Amplifies ORF b129 and flanking sequences
b129 Check R	5′-TTA ATA TAG CTG CGA TGC AGT ATA GTT TAT TTG TGC-3′	Amplifies ORF b129 and flanking sequences

*Underlined sequence indicates ORF.