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Differentiation of rat LG immature cells in epithelial cell media. Immature cells from LG (A) were grown in RPMI media, which selects for epithelial cells for two weeks. Differentiated LG cells then were stained with the markers for LG myoepithelial cells α-SMA (α sm mus actin, [B]), adenylate cyclase II (C), α-actinin (D), and vimentin (E). Rabbit (rab) isotype control is shown in (F). Magnification 400×.
