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. Author manuscript; available in PMC: 2012 Jun 5.
Published in final edited form as: J Immunol. 2010 Oct 11;185(10):5769–5777. doi: 10.4049/jimmunol.1002226

Figure 2. The 10.1.1 antibody recognizes mCLCA1.

Figure 2

A). Immunoprecipitation of spleen or transfected 293 cell lysates with 10.1.1 or hamster antibodies, followed by SDS-PAGE and immunoblotting with 10.1.1 antibody. The 90 kD species is detected after 10.1.1 antibody immunoprecipitation of lysates from spleen, and a slightly smaller protein is detected in immunoprecipitates from 293 cells transfected with mCLCA1, but not from control mAST-transfected 293 cell lysates. Hamster IgG does not immunoprecipitate these proteins. B). SDS-PAGE and immunoblotting with 10.1.1 antibody identifies a 90 kD protein in spleen lysates and a slightly smaller protein in 293 cells transfected with pcDNA-mCLCA1 plasmid (293-mCLCA1), but not with pcDNA-mAST plasmid (293-mAST). Blots were re-probed with antibody to detect 42 kD actin as a loading control.