Figure 3.

Shp1, Reg1 and Glc8 are required for glucose-induced activation of PP1, whereas absence of other subunits has only a partial effect on activation, or an inhibitory effect. At time 0, 20 mM glucose was added to glucose-deprived (glycerol-grown) cells of BY-strains with different deletions and cell extracts were used to measure specific PP1 activity. (A) WT (•), bni4Δ (○), sds22Δ (▴), sip5Δ (△), bud14Δ (▪), gip1Δ (□) and gip2Δ (▾). (B) WT (•), reg2Δ (○), glc8Δ (▴), red1Δ (△) and gac1Δ (▪). (C) WT (•), pig1Δ (○) and pig2Δ (▴). (D) WT (•), shp1Δ (○) and reg1Δ (▴).