Figure 5.
Requirement for Reg1 and Shp1 in glucose-induced activation of PP2A. (A, B and D) At time 0, 20 mM glucose was added to glucose-deprived (glycerol-grown) cells of different deletion strains and cell extracts were used to measure specific PP2A activity. (A) WT (•), reg1Δ (○) and shp1Δ (▴). (B) WT (•), reg1Δ (○), reg1Δ + pReg1 (▴), reg1Δ + pReg1Δ8 (△) and reg1Δ + pReg1I466M F468A (▪). (C) 20 mM glucose was added to glucose-deprived (glycerol-grown) cells of BY-strains with different deletions (WT, shp1Δ and reg1Δ) that are expressing HA-tagged Pph21 or Glc7, respectively. Antibodies used for the immunopurification (IP) and immunodetection (western): HA: anti-HA; Rts1: anti-Rts1; -: no antibody. (D) WT strain (•), pig1Δ (○), pig2Δ (▴), cdc55Δ (△) and pig2Δ cdc55Δ (▪).