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. 2012 Jan 3;22(6):986–1002. doi: 10.1038/cr.2012.4

Figure 1.

Figure 1

Ectopic expression of MLL-AF4 in hESCs activates Hox gene expression. (A) Schematic representation of the lentiviral vectors used. (B) Phase contrast morphology of colonies from NEO- and MLL-AF4 hESCs. (C) RT-PCR confirming expression of the MLL-AF4 transcript in transduced hESCs. Line 1 was used as a housekeeping gene. (D) Western blot detection of the MLL-AF4 protein in transgenic hESCs. 293T cells transduced with MLL-AF4 and MLL-AF4ΔNH2 were used as positive and negative control, respectively. ERK1 and ERK2 blot was used as loading control. (E) Hox gene expression profiling in MLL-AF4 vs NEO hESC using microarrays. The inset shows qPCR validation of the indicated Hox genes/co-factors.