Table 2. Laboratory findings and genotyping of multidrug-resistant isolates from 21 tuberculosis patients, Bizerte, Tunisia, 2001–2004*.
| Patient | Smear result | Resistance pattern† | RFLP‡ | Spoligotype | PGG§ | Mutational analysis |
||||
|---|---|---|---|---|---|---|---|---|---|---|
| rpoB | katG | pncA | mutT3 | Ogt | ||||||
| P1 | +++ | HSREZ | 11 | Haarlem3¶ | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P2 | + | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P3 | ++ | HSREZ | 12 | Haarlem3 | 2 | S531L+V610M | S315T | T11G (L4W) | L209L | T15S |
| P4 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P5 | - | HSREZ | 12 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P6 | + | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P7 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P8 | - | HSRE | 11 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P9 | + | HSRE | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P10 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P11 | - | HSREZ | 12 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P12 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P13 | - | HSRE | ND# | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
| P14 | - | HRZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | G insertion (391-392) | L209L | T15S |
| P15 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | A-11C | L209L | T15S |
| P16 | ++ | HSR | 12 | Haarlem3 | 2 | S531L+V610M | S315T | T11G (L4W) | L209L | T15S |
| P17 | - | HSREZ | 11 | Haarlem3 | 2 | S531L+V610M | S315T | G insertion (296-297) | L209L | T15S |
| P18 | - | HSREZ | 12 | Haarlem3 | 2 | S531L+V610M | S315T | T11G (L4W) | L209L | T15S |
| P19 | - | HSRE | 9 | Other** | 2 | ΔN (AAC)519 | S315T | G insertion (296-297) | WT | WT |
| P20 | ++ | HSR | 10 | Haarlem3 | 2 | S531L | S315 | WT | L209L | T15S |
| P21 | ++ | HR | 11 | Haarlem3 | 2 | S531L+V610M | S315T | WT | L209L | T15S |
*RFLP, restriction fragment length polymorphism; PGG, principal genetic grouping; WT, wild type (identical to strain H37Rv); ND, not determined. †H, isoniazid; S, streptomycin; R, rifampicin; E, ethambutol; Z, pyrazinamide. ‡Number of IS6110 bands. §Principal genetic grouping according to gyrA and katG polymorphisms (11). ¶Absence of spacers 31 and 33–36. #IS6110 typing was determined by ligation-mediated polymerase chain reaction and the profile was identical to the other outbreak-associated strains. **Absence of spacers 15, 21–24, and 33–36.