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. 2011 Nov 1;12(9):818–826. doi: 10.4161/cbt.12.9.17682

Figure 2.

Figure 2

Effects of VMY on DAOY cell proliferation. (A) DAOY cells were treated for 18 h with either DMSO, the dansyl group alone or VMY-1-103 at the concentrations shown. Cells were harvested and cell viability assessed by trypan blue dye exclusion on >300 cells. Data are average ± standard deviation of n ≥ three separate experiments. (B) apoptosis proteome arrays performed on extracts from DAOY cells were treated for 18 h with either DMSO or VMY at 30 uM. Fold change in protein abundance vs. DMSO are shown as Ave ± deviation of duplicate samples from n = two separate experiments. A representative proteomic array is shown at top. (C) Representative protein gel blot (n ≥ 3) performed on DAOY cells treated for 18 h with either PVB or VMY at the concentrations shown. (D) In vitro CDK1-kinase assays performed on DAOY cell extracts treated as marked. Data are percent inhibition in substrate phosphorylation vs. vehicle control for n = two experiments. Flavo, Flavopiridol; Cl- capase-3, cleaved caspase-3; PARPFL, full length PARP; PARPL, 89 kD fragment of cleaved PARP; Noc, nocodazole; Veh, vehicle.