Figure 6.

Merged fluorescent imaging of DAOY cells. Cells were treated DMSO or VMY (30 um) for 1 h. (A and B) Cells were stained with phospho-histone H3 (yellow) and Aurora Kinase A antibodies (red) as well as with DAPI (blue) and phalloidin for F-actin (green). (C and D) Cells were stained with phospho-Wee1 (yellow) and Aurora Kinase A antibodies (red) as well as with DAPI (blue) and phalloidin for F-actin (green). The Wee1/Aurora A merge is in orange. PM, prometaphase; M, metaphase; A, anaphase; T, telophase. Arrows, trailing or misaligned chromosomes, *, mislocalized Wee1, Aurora Kinase A or phospho-histone H3.