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. Author manuscript; available in PMC: 2012 Jun 5.
Published in final edited form as: Pflugers Arch. 2012 Mar 17;463(6):799–818. doi: 10.1007/s00424-012-1093-z

Scheme I. Construction of Kv1 deletion mutants and GST-fusion proteins.

Scheme I

The full-length Kv protein consists of 3 distinct domains: the N-terminus or the T1-domain, a trans-membrane domain (TMD) which forms the ion-conducting pore, and a C-terminal cytosolic loop. Deletion mutants were generated by successive deletion of 18, 37, and 58 amino acids from C-terminal end as indicated to the left of the drawings. In a separate series of experiments, peptides corresponding to the amino acid sequence spanning the last 19, 38, and 60 amino acids were expressed fused with GST.