Skip to main content
. Author manuscript; available in PMC: 2012 Jun 5.
Published in final edited form as: Pflugers Arch. 2012 Mar 17;463(6):799–818. doi: 10.1007/s00424-012-1093-z

Fig. 7. Effect of C-terminal deletions on regulation of Kv currents by pyridine nucleotides.

Fig. 7

COS-7 cells were transfected with Kv1.5WT or KvΔC constructs coexpressed with Kvβ3. Kv currents were recorded with either control internal solution or solution containing NADPH or NADP+ in the patch pipette. Percent inactivation was measured from the peak current to the end of 800 ms pulse at +50 mV. (a) % inactivation measured with no additives (none) or NADPH or NADP+ in the patch pipette. Inset shows the range of regulation by pyridine nucleotides calculated as a difference in the overall percent inactivation between NADPH and NADP+ groups. Rate constants of the fast (b) and the slow (c) phases of inactivation, respectively. The decay phase was analyzed by using mono- or bi-exponential equation (see Material and Methods for detail). Bars are color-coded for the deletion construct. *P<0.05 compared with no-additions (none) within each group, †P<0.05 compared with WT (none), ‡P<0.05 compared with WT (NADPH).