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Selecting coprolites. (a) Coumère cave coprolite. (b) PCR amplification of a fragment of the cave hyena cytb gene. Amplification (33 PCR cycles) was carried out on 0.04 to 2.5% of each DNA extract. (c) Production of libraries of DNA fragments for high-throughput sequencing. Coprolite and mock extracts ligated to oligonucleotide adapters were amplified using 12 PCR cycles. The upper band (specific for coprolite samples) was recovered for Illumina sequencing.
