Fig. 1.

Dyrk1A modulates p120-catenin levels and the intracellular localization of Kaiso. (A) Either Dyrk1A1 or -1A2 was co-injected with HA-tagged p120-catenin (0.25 ng) into Xenopus embryos at the one-cell stage, followed by immunoblotting for HA–p120-catenin (12CA5) or actin (negative control). (B) HA–p120-catenin (0.25 ng) was microinjected into Xenopus embryos with wild-type or kinase-dead (KD-K188R) Dyrk1A. Embryos were harvested as early gastrulas (stage 10–11) and immunoblotted for HA–p120-catenin, with actin serving as an internal loading control. (C) Increasing doses of HA–Dyrk1A were transfected into 293T cells, and endogenous p120-catenin, β-catenin and GAPDH monitored by immunoblotting. (D) HEK293T cells were transfected with one or both Dyrk1A siRNAs (50 pmol), as indicated, for 48 hours. Endogenous p120-catenin, β-catenin, Dyrk1A and GAPDH levels were monitored by immunoblotting (pp120, BD Transduction; Dyrk1A, ab71464, Abcam). Representative outcomes of experiments repeated three or more times with consistent results are shown.