Table 1.
Caveolin-1 Expression in Glioblastoma Multiforme
| Family member tested | Method | Tested sample | Control | Variation observed | Amplitude of the variation | Reference |
|---|---|---|---|---|---|---|
| Caveolin-1 | DNA microarray | Pooled mRNA from 2 GBM | Normal brain tissue mRNA, pooled | Increased | 5.2-fold | 68 |
| Caveolin-1 | Northern blotting and immunoblotting | mRNA and protein lysates from C6 and DITNC1 transformed astroglial cell lines | Rat type 1 primary astrocytes | Increased in C6 but not DITNC1 transformed astrocyte cell lines compared to astrocytes | 2-fold (mRNA) and 1.5-fold (protein) increased in C6 cell line | 70 |
| Caveolin-1, 2, 3 | RT-PCR and Q-PCR | mRNA from GBM cell line U87MG | fetal primary astrocytes | Increased caveolin-1 mRNA in GBM but reduced caveolin-2 and caveolin-3 | ∼1.5-fold increase (cav-1 mRNA) and 2+ fold decrease (caveolin-2 and 3 mRNA) | 69 |
| Caveolin-1 | Q-PCR | mRNA from 6 surgically removed GBM | Two samples of non-malignant brain lesions | Increased caveolin-1 mRNA in GBM tissue | 20–300 fold difference | 69 |
| Caveolin-1 | IHC | 19 GBM | Astrocytoma II and III, normal brain tissue | Increased caveolin-1 immunoreactivity associated with tumor grade - Increased plasma membrane localization in GBM | N/A | 66 |
| Caveolin-1 | Confocal immunofluorescence microscopy and FACS | U251 MG GBM cell line and 4 primary glioma cultures | Punctate staining of caveolin-1at the plasma membrane in primary GBM cells and GBM cell line reminiscent of caveolae | Significantly more caveolin-1 in GBM compared with grade III astrocytoma | 66 | |
| Caveolin-1 | Western blot analysis of tissue lysates | 9 GBM | Normal brain tissue | No statistically significant change in caveolin-1 expression by analysis of variance. | One of the GBM expresses very high amounts of caveolin-1 but not the 8 others. | 104 |
| Caveolin-1 | Immuno-histochemistry | 73 gliomas including 20 GBM | Other gliomas (low-grade astrocytomas, oligodendrogliomas…) | Variability in caveolin-1 staining both in intensity and in quantity of stained cells | Caveolin-1 expressed in more than 50% of the cells in high-grade tumors. Caveolin-1 is expressed in less than 50% of the cells in low-grade tumors. | 67 |
Abbreviation: GBM, glioblastomas.