Table 1.
Detection of FL1+ cells in human low-grade gliomas (WHO grades I-II). The table summarizes the human tumor specimens studied according to an approved institutional protocol and provides relevant clinical information including gender, age, GFAP, and Ki67 (proliferation index). Percentage of FL1+ detected in prospective analysis, the number of passages during which spheres were expanded and remained viable, the relative mean of fluorescence intensity FL1 are also detailed and categorized according to WHO grade
| Specimen | Gender | Age | Type | Grade | Location | % Ki67+ cells | GFAP content | % FL1+ cells (fresh) | SC culture P < 5 | SC culture p5-10 | SC culture P > 10 | FL1 MI < 103 | FL1 MI > 103 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SE 2 | F | 37 | Subependymoma | I | 4th ventricule | 1 | + | ND | + | + | |||
| SE 3 | M | 62 | Subependymoma | I | ND | 3 | + | 0.185 | + | + | |||
| pA 1 | F | 15 | Pilocytic Astrocytoma | I | left hippocampus | 2–5 | + | 1.7 | + | + | |||
| pA 2 | M | 2 | Pilocytic Astrocytoma | I | vermis | 3–9 | + | 1.425 | + | + | |||
| Average | 29 | 3.25 | 1.56 | ||||||||||
| Mean (absolute value) | 3/4 | 4/4 | 0/4 | 0/4 | 4/4 | 0/4 | |||||||
| Mean (percentage) | 75.00% | 100% | 0.00% | 0.00% | 100% | 0.00% | |||||||
| E II-1 | M | 29 | Ependymoma | II | intramedular | <5 | + | 0.35 | + | + | |||
| A II-1 | F | 22 | Fibrillar Astrocytoma | II | frontal left | 3 | + | 1.17 | + | + | |||
| A II-2 | M | 58 | Fibrillar Astrocytoma | II | temporal right | 5 | + | 2.51 | + | + | |||
| A II-3 | M | 30 | Fibrillar Astrocytoma | II | left hippocampus | 3 | ++ | 1.065 | + | + | |||
| OA II-1 | F | 44 | Oligoastrocytoma | II | fronto-orbital right | 3 | + | 1.87 | + | + | |||
| OA II-2 | M | 41 | Oligoastrocytoma | II | ND | 5 | + | 1.29 | + | + | |||
| OA II-3 | F | 29 | Oligoastrocytoma | II | frontal left- peri-insular | 4 | + | 7.42 | + | + | |||
| OA II-4 | M | 35 | Oligoastrocytoma | II | fronto-temporal-insular left | 2–3 | + | 0.01 | + | + | |||
| OA II-5 | F | 44 | Oligoastrocytoma | II | frontal right | 5 | + | 0.07 | + | + | |||
| OA II-6 | F | 74 | Oligoastrocytoma | II | temporal left | 2–4 | + | ND | + | + | |||
| OA II-7 | M | 44 | Oligoastrocytoma | II | frontal left | 2 | + | 3.1 | + | + | |||
| OA II-8 | F | 70 | Oligoastrocytoma | II | insular left | 2–7 | + | 0.46 | + | + | |||
| OA II-9 | M | 47 | Oligoastrocytoma | II | frontal right | 3 | + | 0.71 | + | + | |||
| O II-1 | F | 71 | Oligodendroglioma | II | frontal right | 5–15 | + | 1.76 | + | + | |||
| Average | 46.85 | 4.14 | 1.68 | ||||||||||
| Mean (absolute value) | 13/14 | 13/14 | 1/14 | 0/14 | 14/14 | 0/14 | |||||||
| Mean (percentage) | 92.86% | 92.86% | 7.14% | 0.00% | 100% | 0.00% |
Abbreviation: ND, not determined.