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. 2012 May 9;14(6):745–760. doi: 10.1093/neuonc/nos088

Fig. 6.

Fig. 6.

Downregulation of uPAR and cathepsin B altered c-Met signaling in GICs. (A–B) Interaction of uPAR with c-Met in U87 and 4910 non-GICs and GICs. An immunoprecipitation assay was performed to determine the effect of uPAR, and cathepsin B knockdown on interaction of uPAR with c-Met. Total cell lysates were subjected to immunoprecipitation with uPAR and co-immunoprecipitates of uPARs were analyzed by Western blotting using specific antibodies to c-Met (phospho-Met). Blots are representative of 3 experiments. (C–F) Effect of downregulation of uPAR and cathepsin B on PI3K, AKT, and JNK. Cell lysates were analyzed for expression of total and phosphorylated PI3K, AKT, and JNK in both pCU-treated and pCU + radiation-treated U87 and 4910 non-GICs and GICs by Western blotting. Blots are representative of 3 independent experiments.