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. 2012 May 8;14(6):712–719. doi: 10.1093/neuonc/nos089

Fig. 4.

Fig. 4.

miR-181d regulates MGMT through its 3′UTR. (A) Suppressive effect of miR-181d on MGMT expression in A1207 glioblastoma cells was rescued by the transfection of MGMT cDNA. β-actin was used as a loading control. (B) miR-181d–induced temozolomide sensitivity in the A1207 glioblastoma line was rescued by the transfection of MGMT cDNA. Cell survival was determined by direct cell count after trypan blue staining. The result represents the average of 10 independent fields. The experiment was repeated 3 times. (C) miR-181d–induced temozolomide sensitivity in the LN18 glioblastoma line was rescued by the transfection of MGMT cDNA. Cell survival was determined by clonogenic assay. The experiment was repeated three times. Similar results were observed in A1207, LN340, and T98G cell lines.