Table 1.

Summary of binding constants

Ligand	Analyte	Buffer	kon, M−1 s−1 × 105	koff s−1 × 10−5	KD(kinetics) nM	KD(equilibrium) nM
VWF	VWFpp	HEPES, pH 7.4, 2.5mM Ca2+	1.7	438	25.8	30.9
VWF	VWFpp	MES, pH 6.2, 10mM Ca2+	3.6	7.58	0.21	3.84
VWF	VWFpp	HEPES, pH 7.4, 2.5mM EDTA	NB	NB	NB	NB
VWFpp	VWFpp	HEPES, pH 7.4, 2.5mM Ca2+	NB	NB	NB
VWFpp	VWFpp	MES, pH 6.2, 10mM Ca2+	0.356	100.5	28.1
VWFpp	D′D3	MES, pH 6.2, 10mM Ca2+	0.55	110	20.1
D′D3	VWFpp	HEPES, pH 7.4, 2.5mM Ca2+	1.76	862	49

NB indicates no binding.