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. Author manuscript; available in PMC: 2012 Jul 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Jun 10;50(30):6839–6842. doi: 10.1002/anie.201101157

Figure 2.

Figure 2

Structure of the caged tyrosine 1, and Coomassie-stained SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) of the expression of the photocaged ZFN through incorporation of 1 at position Y471. M: NEB broad-range protein marker. WT: wild-type ZFN expressed from pET24d-NLS-ZFNY471TAG plasmid with MjYRSwt (expressed from pEVOL-wt). +1: caged ZFN expressed from pET24d-NLS-ZFNY471TAG and MjYRSONBY (expressed from pEVOL-ONBY) grown in medium supplemented with 1 (1 mM). −1: caged ZFN expressed from pET24dNLS-ZFNY471TAG and pEVOL-ONBY in the absence of 1.