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. 2012 Apr 6;119(21):4898–4907. doi: 10.1182/blood-2012-01-403089

Figure 3.

Figure 3

Mice undergoing Ott1 deletion are able to maintain peripheral blood counts and BM HSC populations older than 18 months but have reduced colony-forming potential. (A) Automated peripheral blood analysis of samples from 18-month-old Ott1flox/null Mx1-cre (KO) or littermates possessing a WT Ott1 allele (control) pIpC-treated at 6 weeks of age. Control, n = 6; KO, n = 5. (B) Absolute numbers of LT-HSCs (Lin−Sca-1+c-Kit+CD34−), ST-HSCs (Lin−Sca-1+c-Kit+CD34+), progenitors (Lin−Sca-1−c-Kit+), and total BM obtained from 18-month-old Ott1 KO and control mice in panel A. (C) Myeloid CFU ability of BM from 18-month-old Ott1 KO or control mice in panel A. Samples plated in duplicate (control, n = 4; Ott1 KO, n = 3). Gran indicates CFU-granulocyte; GM, CFU-granulocyte/monocyte; Mono, CFU-monocyte; and Ery, CFU-erythroid. Graphs represent mean values, and error bars represent SD.