Table 1. Thermodynamic Parameters for WT IL-1β and All Permutant Variants.

IL-1β	ΔGN-U a,b(kcalmol−1)	ΔΔGN-U a,b(kcalmol−1)	m-valueN-U a,b(kcalmol−1M−1)	Cm c (M)
WT	7.7±0.2	0	6.2±0.3	1.5±0.1
PM23	6.8±0.4	−0.9±0.4	4.8±0.2	1.4±0.1
PM65	4.3±0.3	−3.4±0.4	3.4±0.5	1.3±0.2
PM76	4.0±0.1	−3.7±0.2	2.9±0.3	1.3±0.1
PM142	6.2±0.5	−1.5±0.5	4.8±0.4	1.4±0.2

Changes in folding parameters upon circular permutating IL-1β at various turns and loops. The equilibrium data were fit using MATLAB in order to obtain equilibrium parameters for folding, ΔG_N-U. Changes in ΔG_N-U (ΔΔG_N-U) were obtained using WT as a reference. m-value indicates changes in the accessible surface area upon folding and indicate cooperativity of folding.

^aEquilibrium transition data were evaluated using a two-state folding model.

^bData were obtained by calculating the average wavelength and calculating the relative average wavelength in terms of F_app as a function of [Gnd-HCl].

^cC_m values were taken from dividing the ΔG_N-U by the m-value.