Table 1. Locality data for all individuals examined.
| site no. | Locality | station code | species | cytb N | S7 N | allozyme N |
| 1 | Bray Drain, Robe Naracoorte Rd, SA | FISH99 | G. pusilla-west | 9 | 1 | 9 |
| 2 | Bakers Range, SA | FISH90 | G. pusilla-west | 11 | 1 | 11 |
| 3 | Drain 32 outside Millicent, SA | FISH99 | G. pusilla-west | 9 | 1 | 10 |
| 4 | Drain into Lake Letty (Dismal Swamp), SA | FISH90 | G. pusilla-west | 5 | 1 | 5 |
| 5 | Swamp on entrance rd to Piccaninnie main pond, SA | FISH99 | G. pusilla-west | 10 | 1 | 10 |
| 6 | Wannon R near Dunkeld, VIC | PU02-117 | G. pusilla-west | 10 | 1 | 10 |
| 7 | Darlot Ck near Homerton, VIC | PU02-114 | G. pusilla-west | 10 | 1 | 10 |
| 8 | Merri R, Grassmere, VIC | PU02-111 | G. pusilla-west | 6 | 1 | 6 |
| 9 | Mount Emu Ck, Panmure, VIC | PU02-112 | G. pusilla-west | 2 | 1 | 2 |
| 10 | Gosling Ck near Murroon, VIC | PU02-87 | G. pusilla-west | 9 | 1 | 9 |
| 11 | Tirhatuan Swamp (now at LaTrobe University), VIC | PU03-03 | G. pusilla-east | 10 | 1 | 10 |
| 12 | Tuerong Ck, VIC | PU03-04 | G. pusilla-east | 11 | 11 | |
| 13 | Cardinia Ck, Beaconsfield, VIC | PU02-102 | G. pusilla-east | 10 | 1 | 10 |
| 14 | Yallock Ck, Koo Wee Rup, VIC | PU02-103 | G. pusilla-east | 10 | 1 | 10 |
| 15 | Off Five Mile Track, Wilsons Prom, VIC | PU02-71 | G. pusilla-east | 9 | 1 | 10 |
| 16 | Ck from Freshwater Lake, Wilsons Prom, VIC | PU02-70 | G. pusilla-east | 1 | 1 | 1 |
| 17 | Moe R drains, Moe, VIC | PU02-80 | G. pusilla-east | 10 | 1 | 10 |
| 18 | Perry R at Princess Hwy, VIC | PU02-68 | G. pusilla-east | 6 | 1 | 6 |
| 19 | Swamp near Harcus, TAS | FISH98 | G. pusilla-east | 10 | 1 | 9 |
| 20 | Gladstone Lagoon near Gladstone, TAS | FISH98 | G. pusilla-east | 2 | 1 | 2 |
| 21 | Tributary to Icena Ck on rd to Ansons Bay, TAS | FISH98 | G. pusilla-east | 10 | 1 | 10 |
| 22 | West of Five Hill Rd, Flinders Island, TAS | FISHY4 | G. pusilla-east | 11 | 11 | |
| 23 | Lennard Brook, WA | G. munda | 1 | |||
| 24 | Melaleuca Park, Wetland EPP173, WA | G. nigrostriata | 3 | 1 | ||
| 25 | Kemerton Nature Reserve, WA | G. nigrostriata | 3 | 2 | ||
| 26 | Ironstone Gully, Buayanyup R, WA | G. munda | 3 | 2 | ||
| 27 | Canebreak Pool, Margaret R, WA | PU09-58 | G. munda | 2 | ||
| 28 | Roadside pools off Scott R Rd, WA | G. nigrostriata | 2 | 1 | ||
| 29 | Rosa Brook, Mowen Rd, WA | PU09-57 | G. munda | 2 | ||
| 30 | Milyeannup Brook, Brockman Hwy, Blackwood R, WA | PU09-53 | G. munda | 1 | ||
| 31 | Tributary of Donnelly R, upstream of Scott Rd, WA | PU09-52 | G. munda | 2 | 1 | |
| 32 | Pemberton, WA | GenBank | G. munda | JN232599.1 | JN232707.1 | |
| 33 | Doggerup Ck, Windy Harbour Rd, WA | PU09-49 | G. nigrostriata | 2 | 1 | |
| 34 | Chesapeake Rd west, WA | G. nigrostriata | 2 | 2 | ||
| 34 | Chesapeake Rd west, WA | GenBank | G. nigrostriata | NC_008448.1 | ||
| 35 | Boorara Brook, Muirillup Rd, WA | PU09-48 | G. munda | 2 | 1 | |
| 36 | Moores Hut Track, WA | G. nigrostriata | 2 | 1 | ||
| 37 | Shannon R, South Western Hwy, WA | PU09-47 | G. munda | 2 | 1 | |
| 38 | Deep R, Beardmore Rd below Fernhook Falls, WA | PU09-45 | G. munda | 2 | 1 | |
| 39a | Mitchell R, Denmark-Mt Barker Rd, WA | PU09-37 | G. munda | 2 | 1 | |
| 39b | Mitchell R, WA | G. munda | 1 | |||
| 40 | Marbelup Brook, off Marbelup North Rd, WA | PU09-38 | G. munda | 2 | 1 | |
| Talka, Maule Basin, Chile | GenBank | B. gothei | JN232601.1 | JN232709.1 | ||
| Tributary to Laguna Saval, Valdivia Basin, Chile | GenBank | B. bullocki | JN232602.1 | JN232710.1 | ||
| (LU) | 8 km N of Los Ulmos, Valdivia Basin, Chile | DAN04-24 | B. bullocki | 1 | 1 | |
| (RN) | Rio Negro, Maullin Basin, Chile | B. bullocki | 2 | 2 |
The site number refers to the location of each sample site as shown on Figure 1. The location column gives the general location of each sample. Station code refers to the field number or SAMA EBU code that samples are catalogued as. The last three columns provide the number of individuals (N) examined for each marker. Abbreviations: Ck, creek; Hwy, Highway; R, river; Rd, road; SA, Southern Australia; TAS, Tasmania; VIC, Victoria; WA, Western Australia.