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. 2012 May 1;125(9):2172–2184. doi: 10.1242/jcs.096214

Fig. 4.

Fig. 4.

Mapping the interaction between β-tails and kindlins using GST pull-down assays. (A) Sequences of the β1 and β6 cytoplasmic tails used to generate the GST constructs. Pull-down of endogenous kindlin-1 (B) and kindlin-2 (C) with GST–β1, GST–β6, GST–β6Δ and GST alone. Pull-down of FLAG-tagged kindlin-1 with GST–β1, GST–β6, GST–β6Δ and GST (D) and FLAG-tagged kindlin-2 with GST–β1, GST–β6, GST–β6Δ, GST–β1(K974A), GST–β1(Y795A) and GST (E) expressed in CHOA5 cells. Pull down of FLAG tagged kindlin-1 (F) and FLAG-tagged kindlin-2 (G) with GST–β1, GST–β6, GST–β1-β6, GST–β6-β1 and GST. (F). Pull down of FLAG tagged kindlin-1 (H) and FLAG-tagged kindlin-2 (I) with GST–β1, GST–β6Δ, GST–β6Δβ1(NPKY), GST–β6Δβ1(KSA) and GST. (F). Quantification of the amount of FLAG-tagged kindlin-1 (J) and kindlin-2 (K) pulled down by the various GST constructs. Results are normalized for loading against GST control (NIH ImageJ software). Data show means ± s.e. from three independent experiments.

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