Fig. 5.

Expression of kindlin-2 is important for cell spreading in keratinocytes. (A) Western blot analysis of lysates from cells expressing scrambled and kindlin-2 shRNA with kindlin-2 and kindlin-1. Tubulin was detected as a loading control. (B) Box plot of the distribution of the average cell area along the median (n=100) of scrambled and kindlin-2-knockdown cells (***P<0.001, compared with scrambled cells). Scrambled (C,C′) and kindlin-2-knockdown cells (D,D′) stained with β1 (green) and kindlin-2 (red). Scrambled (E,E′) and kindlin-2-knockdown cells (F,F′) stained with β6 (green) and kindlin-1 (red). Scrambled (G,G′) and kindlin-2-knockdown cells (H,H′) stained with vinculin (green) and phalloidin (red). Scale bar: 10 μm.