Fig. 6.

Rescue of β1-KO cell spreading correlates with targeting of kindlin-2 to focal adhesions. Surface expression of endogenous β1 on WT (A) and KO (D) cells assessed by FACS. (B,C) WT cells stained for β1, vinculin and kindlin-2, as indicated. (E,F) KO cells stained for β1, vinculin, β6 and kindlin-2, as indicated. (G–R′) Surface expression of β1 on KO cells rescued with a full-length β1 (G), β1-β6 (J), β1–β1-β6 (M), or β1–β6-β1 (P) was assessed by FACS. Cells rescued with full-length β1 (H,I), β1-β6 (K,L), β1–β1-β6 (N,O), or β1–β6-β1 (Q,R) were stained for β1, vinculin and kindlin-2, as indicated. Scale bar: 10 μm.