Fig. 2.
Cofilin plays a role in the regulation of respiration and ROS production. Cells were grown in triplicate for 24 hours to diauxic shift in YPD. (A) Mitochondrial respiration was measured in a known number of whole cells and (B) ROS accumulation was assessed using the indicator dye H2DCF-DA by flow cytometry. (C) Superoxide dismutase activity present in protein extracts from wild type, cof1-6, cof1-10, cof1-13 and cof1-22 (Sod1p and Sod2p) strains grown for 24 hours to diauxic shift in YPD were assessed after separation on separated in a native gel. Statistical significance with reference to wild type was assessed using a Student's t-test, *P<0.05, **P<0.01.