Fig. 6.

Cofilin-mediated azole resistance is dependent on the ABC transporter and requires the transcription factor PDR1. (A) The biosynthesis of ergosterol was assessed in log and post diauxic shift wild-type, cof1-5, cof1-6, cof1-10 and cof1-22 cells by measuring pathway intermediates using a Gas-chromatography/Mass spectrometry approach as described in the Materials and Methods. (B) The effects of adding the Pdr5p inhibitor FK506 (10 μg/ml) upon the sensitivity of cof1-6 cells to itraconazole (4.4 μM) was observed in liquid medium. (C) The influence of the transcription factors PDR1 and PDR3 upon resistance to itraconazole (4.4 μM) was investigated by comparing growth of wild-type, cof1-6, cof1-6Δpdr1 and cof1-6Δpdr3 cells.