Figure 4. Inositol phosphate accumulation in COS-7 cells expressing wild type GNRHR1 and the two hGNRHR1 mutants, Pro282Arg and Tyr323Cys.

COS-7 cells were transfected with the expression vector indicated and then grown in the presence of pharmacoperone (NBI-42902) or vehicle (DMSO) and then washed before the addition of myo-[2-³H]Inositol. After 16 h the cells were washed again and then incubated for 1 h with graded concentrations of GnRH1. The amount of intracellular accumulation of labeled inositol phosphates in the two mutant expressing COS-7 cells was compared to cells expressing the wild type receptor.