Table 3. In vitro [3H]-Inositol Phosphate Accumulation Assay.
| +NBI-42902 | ||||
| pEC50 (nM) | Emax | pEC50 (nM) | Emax | |
| Wild type | 9.16±0.12 (0.69) | 100±4 | 9.20±0.23 (0.63) | 115±9 |
| Glu90Lys | ns | ns | 8.82±0.14 (1.51) | 59±3* |
| Pro282Arg | ns | ns | ns | ns |
| Tyr323Cys | ns | ns | 8.76±0.43 (1.74) | 16±3* |
IP responses (pEC50) were measured in COS-7 cells transfected with wild type and mutant receptors.
E max = % of maximal IP responses were expressed relative to wild type receptor without NBI-42902.
ns
No measurable stimulation.
Values are mean ± SEM of three or more independent experiments, converted to nM (in parentheses).
Significant difference of NBI-42902 treatment on E max is represented by an asterisk * (p<0.05).