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. 2012 Jun 5;7(6):e38787. doi: 10.1371/journal.pone.0038787

Figure 6. Effect of G1 on eNOS enzymatic activity in endothelial cells.

Figure 6

(A) The endothelial cells were cultured in vitro, and treated with G1 (10 nM) for 5, 10, 25, 60, 180 min. G1 activated eNOS phosphorylation after 5–10 min treatment. The increased level was maintained up to 30 min, followed by a reduction at 1 h. ** p<0.01 compared with control. (B) Endothelial cells were exposed to high glucose concentration (33 mM) for 48 or 72 h, and then incubated with G1(10 nM) for 10 min. G1 treatment (10 nM, 10 min) reversed the down-regulation of phosphorylation level of eNOS by high concentration of glucose. * p<0.05 compared with the glucose treated before; # p<0.05 compared with the glucose treated at 48 h or 72 h without G1. Results are given as the mean ± SEM of three independent experiments.