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. 2012 Jun 5;7(6):e37615. doi: 10.1371/journal.pone.0037615

Figure 2. Autonomous inhibition of Notch signaling suppresses non-cell autonomous ex-lacZ in vps25 mosaics.

Figure 2

Shown are MARCM-induced vps25 mosaic wing discs expressing the transgenes indicated. vps25 mutant cells are marked in green. ex-lacZ is detected by β-Gal labeling (red or grayscale). Arrows point to representative examples. (A,A’,B,B’) Autonomous expression of dominant negative bsk (bskDN; A,A’) (89% of wing discs showed non-cell autonomous ex-lacZ (n = 9)) and domeless (domeDN; B,B’) (82% of wing discs showed non-cell autonomous ex-lacZ (n = 28)) does not suppress the non-cell autonomous induction of ex-lacZ in vps25 mosaic discs. (C,C’) Autonomous expression of dominant negative Notch (NotchDN) suppresses the non-cell autonomous induction of ex-lacZ in vps25 mosaic discs (8% of wing discs showed non-cell autonomous ex-lacZ (n = 12)). (D,D’) Autonomous RNAi-induced knockdown of Delta (DeltaRNAi) does not suppress the non-cell autonomous induction of ex-lacZ in vps25 mosaic discs (75% of wing discs showed non-cell autonomous ex-lacZ (n = 12)). (E) Summary of the effects on non-cell autonomous ex-lacZ when JNK, Jak/STAT or Notch activity are autonomously inhibited in vps25 mosaic wing discs. At least 10 discs were assayed/genotype. Genotypes: (A,B) yw hs-FLP/UAS-bskDN or UAS-domeDN; FRT42D Tub-Gal80/ex697 FRT42D vps25N55 y+; Tub-Gal4, UAS-CD8-GFP/+ (C) yw hs-FLP; FRT42D Tub-Gal80/ex697 FRT42D vps25N55 y+; Tub-Gal4, UAS-CD8-GFP/UAS- NotchDN (D) yw hs-FLP; FRT42D Tub-Gal80/ex697 FRT42D vps25N55 y+; Tub-Gal4, UAS-CD8-GFP/UAS-DeltaRNAi.