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. 2012 Jun 5;7(6):e37615. doi: 10.1371/journal.pone.0037615

Figure 3. Activation of Notch signaling is sufficient to induce Yorkie activity.

Figure 3

Shown are MARCM-induced mosaic wing discs (A,B,C, E) expressing the indicated transgenes in otherwise wild-type clones marked in green. (D) expresses NICD under dpp-Gal4. ex-lacZ is detected by β-Gal labeling (red or grayscale). Arrows point to representative examples. (A,A’,B,B’) Overexpression of hepCA (0% of wing discs showed non-cell autonomous ex-lacZ (n = 13)) and upd (0% of wing discs showed non-cell autonomous ex-lacZ (n = 11)) does not lead to non-cell autonomous induction of ex-lacZ. (C,C’) Overexpression of NICD leads to non-cell autonomous induction of ex-lacZ (91% of wing discs showed non-cell autonomous ex-lacZ (n = 23)). (D,D’) Expression of NICD using dpp-Gal4 induces strong non-cell autonomous upregulation of ex-lacZ in the hinge and notum, but not the wing pouch. (E,E’) Overexpression of NICD leads to non-cell autonomous accumulation of Diap1 protein. (F) Summary of the effects on non-cell autonomous ex-lacZ when JNK, Jak/STAT or Notch activity are autonomously induced in wild-type mosaic wing discs. At least 10 discs were assayed/genotype. Genotypes: (A,B) yw hs-FLP; FRT42D Tub-Gal80/ex697FRT42D y+; Tub-Gal4, UAS-CD8-GFP/UAS- hepCA or UAS-upd (C) yw hs-FLP; FRT42D Tub-Gal80/ex697FRT42D y+; Tub-Gal4, UAS-CD8-GFP UAS-NICD (D) ex-lacZ; dpp-Gal4 UAS-GFP/UAS-NICD (E) yw hs-FLP; FRT42D Tub-Gal80/ex697FRT42D y+; Tub-Gal4, UAS-CD8-GFP/UAS-NICD.