Skip to main content
. 2012 Jun 5;7(6):e38464. doi: 10.1371/journal.pone.0038464

Figure 1. Silencing CO-029 expression.

Figure 1

The CO-029 shRNA and nonsilencing shRNA were stably expressed in HT29 cells. (A) Flow cytometric analysis of CO-029 expression at the surface of HT29 cells that were transfected with nonsilencing (NS) or CO-029 shRNA (KD) constructs. The negative control mAb was murine IgG, and CO-029 mAb was NS1116. Mean fluorescent intensity (MFI) of CO-029 on KD cells was 62% less compared to that on NS cells. (B) Western blot analysis of CO-029 proteins in NS and KD HT29 cells. KD cells displayed a 90% of reduction in CO-029 proteins. β-actin: loading control.