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. 2012 Jun 5;10(6):e1001337. doi: 10.1371/journal.pbio.1001337

Figure 2. A conditional transgenic RNAi test of AC involvement in PDF signaling in M cell pacemakers.

Figure 2

(A) Temperature sensitive gal80 was used to induce knockdown in adult cells only. Flies were raised at 18°C and moved to 29°C for 6 h (inactive), to allow for readable levels of Epac1camps sensor, or >36 h (active). Adult induction of AC3RNAi (gal80ts;AC3RNAi (active)) significantly reduces the PDF response. Adult induction of AC76ERNAi shows no significant difference from control. (B) Genetic confirmation of AC3 involvement was performed using two independently generated RNAi lines against AC3 (GD:AC3 and TRiP:AC3) as well as flies that are deficient for the AC3 gene region (Df(2L)DS6). (C) DH31 responses in M cells from flies with a knockdown of AC3 in combination with Df(2L)DS6. All genotypes include Pdf-gal4;Epac1camps. Error bars denote SEM. *** p<0.001 (compared with control).