Table 1. List of primers and genes flanking five intergenic spacers herein studied in relapsing fever Borrelia.
| Spacers | Start End | Spacer flanking genes (5------3) | Primers | PCR product size (bp) | Spacer size (bp) |
| MST2 | 786480.. 786968 | penicillin-binding protein//uncharacterized conserved protein | F:TTTTTGCTAAAATTAACCCTTTTCAR:CTCATTTTAATTTCCTTACCCCTA | 578 | 487 |
| MST3 | 669736.. 670279 | N-acetylmuramoyl-L-alanine amidase, putative//vacuolar X-prolyl dipeptidyl aminopeptidase I | F:GCAGGTGGCTGTTAACCACTR:ATGTGGGGAATGCACTCTTT | 687 | 543 |
| MST5 | 565860.. 566397 | translation elongation factor G//uncharacterized conserved protein | F:CCTGAGTCGATATGGGCACTR:CAACCTGACATATCTTACTCAATTCAT | 653 | 536 |
| MST6 | 494656.. 494903 | tRNA-ser//DNA polymerase III subunits gamma and tau | F:GGGTTCGAATCCCATTTTCTR:CTCTGGGACGCCTCTTAATG | 333 | 246 |
| MST7 | 458283.. 458778 | 16S ribosomal RNA//hypothetical protein | F:TTCGCCACTGAATGTATTGCR:TGCCAATGTTCTTGTTGGTC | 738 | 494 |
Start and end of spacer are according to B. duttonii genome.