Figure 1.

Distance travelled on Saline Day1 (◆), Saline Day 2 (■) and Meth Day 3 (▲) in the F2 female (a) and male (b) mice; as well as in F8 female (c) and male (d) mice. For both the F₂ and F₈ mice, distance travelled on day 1 was slightly greater than on day 2 (p < 0.005) and distance traveled on day 3 was dramatically greater than on either day 1 or 2 (p < 0.0001). The F₂ and F₈ generation also differed from each other on day 1 (p < 0.0001) and day 3 (p = 0.014), but not day 2. Additionally, F₂ and F₈ AIL mice displayed sex and time differences for all three sessions. In the F₂ mice, a two-way ANOVA reported varying effects of sex and time-point on distance travelled for saline day 1 (sex: F_{1, 676} = 56.2; p = 0.0007, time: F_{5, 676} =375.3; p < 0.0001), saline day 2 (sex: F_{1, 676} =18.6; p = 0.008, time: F_{5, 676} = 674.1; p < 0.0001) and methamphetamine day 3 (sex: F_{1, 676} = 5.0; p = 0.08, time: : F_{5, 676} = 273.2; p < 0.0001). A two-way ANOVA also reported significant effects of sex and time-point on distance travelled in the F₈ AIL mice for saline day 1 (sex: F_{2, 552} = 17.2; p = 0.009, time: F_{5, 552} = 336.4; p < 0.0001), saline day 2 (sex: F_{2, 552} = 30.9; p = 0.003, time: F_{5, 552} = 335.9; p < 0.0001) and methamphetamine day 3 (sex: F_{2, 552} = 11.4; p = 0.02, time: F_{5, 552} = 95.7; p < 0.0001).