Fig. 3.

TIMP-2 concentrations in conditioned medium isolated from MCF-7 cells transfected with TIMP-2, ALA + TIMP-2 or GFP. a Known concentrations of recombinant TIMP-2 were assayed using a TIMP-2 ELISA kit and the optical density of each was plotted against thcphe concentration of TIMP-2. b Levels of TIMP-2 in the conditioned media isolated from MCF-7 cells transfected with GFP, TIMP-2 or ALA + TIMP-2 constructs. TIMP-2 levels were determined to be 0.1, 22 and 22 ng/ml in the media from GFP, TIMP-2, or ALA + TIMP-2 transfected cells respectively (as determined by comparing the optical density to the standard curve)