Fig. 5.

Invasiveness and metalloproteinase activity of T47D and MDA-MB-231 cells treated with TIMP-2 or ALA + TIMP-2 conditioned media. a-top) T47D cells treated with 22 ng/ml TIMP-2 conditioned media did not significantly increase invasiveness whereas T47D cells treated with 22 ng/ml ALA + TIMP-2 resulted in a 66% decrease in invasiveness. a-bottom) MDA-MB-231 cells treated with 22 ng/ml TIMP-2 or ALA + TIMP-2 conditioned media resulted in a greater than 80% reductions in invasiveness compared to control. b-top) T47D conditioned media displayed a 45% increase in metalloproteinase activity with the addition of TIMP-2 and a 35% decrease in metalloproteinase activity with the addition of ALA + TIMP-2. b-bottom) Decreases in MDA-MB-231 invasiveness with treatment with TIMP-2 or ALA + TIMP-2 were concomitant with greater than 40% and 50% decreases in metalloproteinase activity respectively. c Conditioned media from control, TIMP-2 or ALA + TIMP-2 treated cells were assayed for MMP activity using zymography. c-top) T47D conditioned media displayed consistent levels of pro-MMP-2 regardless of treatment. c-bottom) MDA-MB-231 conditioned media displayed consistent levels of both pro-MMP-2 and pro-MMP-9, regardless of treatment. a and b–all data are mean ± s.e.m based on 3 replicates. * represents a significant difference between the treatment and the control, while ** represents significant differences between treatments (p < 0.05). C is a representative zymography with several repeats showing consistent levels between treatments