. 2011 Dec 31;17(4):423–433. doi: 10.1007/s12192-011-0317-z

Table 1.

Primer sequence and corresponding annealing temperature of genes

Gene	Primer name	Direction	Primer sequence(5′–3′)	Expected size (bp)	annealing temperature (°C)
GPx	GPx-RTF1	Forward	TGCAACCAGTTCGGGCACCA	173	69
GPx	GPx-RTR1	Reverse	AGTGGCAGTCGCTCCTTCAGGT	173	69
CAT	CAT-RT-F	Forward	ACTCCCATTGCTGTTCGT	114	58.5
CAT	CAT-RT-R	Reverse	ATCCCAATTTCCTTCTTCTG	114	58.5
Prx	Prx-RT-F	Forward	GCTTGCTCTACAGACTCCC	295	58.5
Prx	Prx-RT-R	Reverse	ATACTTCACCGTGCTCATC	295	58.5
18S rRNA	18S2F	Forward	TATACGCTAGTGGAGCTGGAA	147	55
18S rRNA	18S2R	Reverse	GGGGAGGTAGTGACGAAAAAT	147	55