Fig. 2.

Effect of TMDPT and TMDCPT on H₂O₂-induced disruption of neurite outgrowth in differentiated PC12 cells. a The criteria of PC12 differentiation is shown on two neurons (left image) of a sample image. The “P” on the right image indicates the primary neuritis of a neuron 1. The yellow arrow shows the length of a neurite, extent elongated, and membrane-enclosed protrusions of cytoplasm. The green circle on the right image shows the cell body. Neurite width is not equal in all parts of the neurons, thus the average neurite width must be calculated by dividing cell body area to average neurite length. The blue arrows show bipolar cells. The letter “N” indicates the nodes, the sites at which individual neurites branched or separate neurites contacted each other. b NGF-differentiated PC12 cells were pretreated with TMDPT and TMDCPT (10 μM) in the presence of H₂O₂ (150 μM). Criteria were quantified at 24 h; c cell body area; d average neurite length; e average neurite width; f number of primary neurites per cell; g percent of bipolar cells; and h the ratio of nodes to primary neurites. ^#P < 0.05 and ^##P < 0.01, significantly different from control cells. ^*P < 0.05 and ^**P < 0.01, significantly different from H₂O₂-treated cells