Fig. 3.

Hsp90, Hsp70, and Hsp32 levels in PC12 cells pretreated with TMDPT and TMDCPT. a PC12 cells were pretreated with triazine derivatives (10 μM) for 3 h and then exposed to H₂O₂ for 24 h. Twenty-microgram proteins were separated on SDS-PAGE, western blotted, probed with anti-Hsp90, anti-Hsp70, and anti-Hsp32 antibodies, and reprobed with anti-β-actin antibody (one representative western blot was shown; n = 3). The densities of Hsp90 (b), Hsp70 (c), and Hsp32 (d) bands were measured and the ratio was calculated. The mean of three independent experiments is shown. ^#P < 0.05, significantly different from untreated cells. ^*P < 0.05 and ^**P < 0.01, significantly different from H₂O₂-treated cells