Fig. 4.

Effect of TMDPT and TMDCPT on Nrf2 nuclear translocation in PC12 cells. Cells were grown in chamber slides and treated with/without triazine derivatives (10 μM) for 3 h, followed by exposure to H₂O₂ for 24 h. a Nrf2 subcellular localization was determined as described in “Materials and methods”. Immunofluorescent analysis was performed to measure the effects of triazine derivatives on nuclear level of Nrf2 in PC12 cells. b Twenty-microgram proteins were separated on SDS-PAGE, western blotted, probed with anti-Nrf2 antibody, and reprobed with anti-lamin B2 antibody (one representative western blot was shown; n = 3). c The densities of Nrf2 bands were measured and the ratio was calculated. The mean of three independent experiments is shown. ^#P < 0.05, significantly different from untreated cells. ^*P < 0.05 and ^**P < 0.01, significantly different from H₂O₂-treated cells