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Primers used for degenerate and RACE PCRs
| Step | Oligo name | Sequence (5′–3′) |
|---|---|---|
| Degenerate PCR | DP-hsp70.F | CTTAYGGGTTSGACAAGAA |
| DP-hsp70.R | ATCTTGGGGATRCGRGTGG | |
| DP-hsp90.F | GAGACCTTYGCVTTYCAGG | |
| DP-hsp90.R | GTTDCCCACYCCRAAYTGNCC | |
| RACE | GSP-hsp70.3′F | GTACCTTTGATGTCTCTCTGCTCACC |
| GSP-hsp70.5′R | CGGGCTTCAGGGTTCCACGGAACAGGTCCA | |
| GSP-hsp90.3′F | CGCGTTCCAGGCCGAGATTGCACAGCTT | |
| GSP-hsp90.5′R | ACTAATATCAGCTCCGGCCTGAAGAG | |
| AP | CCATCCTAATACGACTCACTATAGGGC |
AP adaptor primer, DP degenerated primer, GSP gene specific primer
