Figure 4. Increased production of TNF-α and IL-6 from lung cDCs and macrophages in the absence of pDCs.
(A) Peak TNF-α(left) and IL-6 (right) production detected by intracellular cytokine analysis from pDCs before and during early (day 1-3) or late (day 4-6) acute influenza virus infection in pDC-intact mice. (B) Representative dot plots of TNF-αand IL-6 production from MHC class II+ CD11c+ cells post-infection. (C) Intracellular TNF-α(left) and IL-6 (right) production from cDCs (top), exudate macrophages (middle) and alveolar macrophages (bottom) from pDC-intact (black bars) and pDC-depleted mice (grey bars) before infection and during early (day 1-3) or late (day 4-6) infection. Shown are means ± SEM. * P<.05 between groups.